MicroscopeMaster.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means to earn fees by linking to Amazon.com and affiliated sites. %%EOF , an amazing new view into the world can be seen with the simple act of placing a sample on a glass slide. When preparing a wet mount specimen for viewing it should be covered with? Place the slide on the stage of a microscope, taking care not to disturb the cover slip. M for the first step followed by O for the second step and U, N, and T for the next steps. Collect your specimen: choose a thin, flat specimen like a plant leaf, insect wing, or single-cell organism. Carefully lay the coverslip on the wet mount. Place the slide on the microscope stage and observe the sample under the microscope. . Forgive my haste. This helps in better observation. Gently tap the cover slip so that the water spreads evenly over the tissue and the edges of the cover slip. Prepare a glass slide by wiping it clean with a lint-free cloth. For samples such as blood, begin by backing the smearing slide into the sample and then push across the slide, pulling the blood in the opposite direction to create a smooth layer. 1 What are the steps to make a wet mount? Biological waste container and bag 4. [L}FvtD_?\Yal{6B7o9H7 Once the iodine or methylene blue has drawn all the way under the slide cover, the specimen is fully dyed. over on top of the drop. Enter a Melbet promo code and get a generous bonus, An Insight into Coupons and a Secret Bonus, Organic Hacks to Tweak Audio Recording for Videos Production, Bring Back Life to Your Graphic Images- Used Best Graphic Design Software, New Google Update and Future of Interstitial Ads. Iodine and methylene blue are poisonous and should never be ingested. ZaneThePwner. 3.2, step 3). Helmenstine, Anne Marie, Ph.D. "How to Prepare Microscope Slides." take the utmost precaution and care when performing a microscope step 1: Place your specimen in the centre of the slide. There are four common ways to mount a microscope slide as described below: In a dry mount, the specimen is placed directly on the slide. wikiHow is where trusted research and expert knowledge come together. Mulch will help retain moisture and help to suppress weeds. Instead of focusing on the edge of the drop, however, you may find it helpful to focus first on the left-hand edge of the coverslip. Approved. Place the material on a clean glass slide. changes over time. Mix well on the slide. This combination was evaluated for finding differences in the details and clarity of morphology and internal structures of parasites under low- and high-power microscopy . Mark the new pause time. x}X[> OHI K l0?0?b!I4ij!Io=I%:N3O;1fLk|Sfi:6u$q2{/kABH Collect the material for the slide. 3.2, step 1). Scrape your thumb carefully on each edge of the coverslip How to Prepare a Wet Mount of Cheek Cells Before starting, it's always important to ensure that the working surface is clean and that you are wearing a pair of clean gloves to avoid contamination. endstream endobj startxref Your paper "specimen" is far too large, it should be smaller than the cover slip. Lower the cover slowly, avoiding air bubbles. A stain can often be applied directly to the specimen before covering with a cover slip. In these circumstances, adding ground pieces of cover glass to the water before the slip layer will create added space and chemicals or strands of cotton can be added to slow the movement of paramecium, amoeba and ciliates. Cover the slide with a coverslip and examine it under the microscope. Make sure the coverslip is clean and free of dirt, dust, or grease. Step Two: Place a drop of liquid on the slide. 0 Manage Settings Make sure to spread it evenly across the surface and to avoid overlapping the edges. Film, using the storyboard to guide you. Hold the cover glass on one side with the help of tweezers. Practice this until you get used to it. Allow the liquid to spread out between the two . When placing a cover slip, there is no adhesive needed. If you would like to change your settings or withdraw consent at any time, the link to do so is in our privacy policy accessible from our home page.. A thicker slide can be created with two drops, but only with the blood of mammals as the erythrocytes lack a nucleus allowing cells to be amassed in multiple layers. Retrieved from https://www.thoughtco.com/how-to-prepare-microscope-slides-4151127. In this method, a drop of culture is placed on a coverslip that is encircled with petroleum jelly (or any other sticky material). Living specimens do not survive long in the heat from an intense microscope Wet Mount. This process is known as chemoorganotrophy. This will reduce the risk of air bubble formation when you position the cover slip. The top and/or bottom edges of a slide can be frosted, enabling easy marking for sample identification and/or orientation. hb```N{>c`0pbj\>1A~: Be sure to Procedure of KOH Wet Mount Preparation. . These solutions may be used to increase contrast in wet or dry mounts. Include your email address to get a message when this question is answered. How do you make a wet mount microscope slide? If youre studying single-celled organismse.g., an amoeba or parameciumtweezers will do little good. B) 1. hbbd``b`$gs $A 5"t( P H\]P c`$ ' experienced with microscopes, and this article ticks all the boxes for correct procedures, tips and warnings. If you work with microscopes or are planning to start, you should learn how to make a wet mount microscope slide. In a wet mount, a drop of water is used to suspend the specimen between the slide and cover slip. Your objective is to have sufficient water to fill the space between cover slip and slide. Place a cover slip onto the microscope slide so that it covers the thin film of liquid culture. Some organisms (like Paramecium) need more space than what forms between a coverslip and a flat slide. endstream endobj 76 0 obj <>stream 89 0 obj <>/Filter/FlateDecode/ID[<805C463A0618AA4683A03A421F488943><2F25A7CD4100F14C97045E7430F981BF>]/Index[72 41]/Info 71 0 R/Length 91/Prev 216683/Root 73 0 R/Size 113/Type/XRef/W[1 3 1]>>stream Read more here. A drop of the solution is added to the liquid drop before applying the coverslip. , Number 1 covers are .13-.17mm thick. Step 4 Evenly smearing the sample across the slide makes it possible to distinguish individual cells. Dry mounts are suitable for specimens such as samples of pollen, hair, feathers or plant materials. Using a pipette or bulb pipette, transfer some of the urine onto a clean glass slide. If the sample won't stay on the slide, it may be secured by painting the slide with clear nail polish immediately before adding the specimen. These more expensive microscope slides can be used without a cover. A vaginal wet mount, also called a wet prep or vaginal smear, is a test done to find the cause of vaginitis, an inflammation of the vagina. Section mounts are suitable for useful for a wide variety of samples such as fruit, vegetables and other solids that can be cut into small slices. The first step in making your own slides is to choose the type of mount you want. Once the nail polish has dried, gently dip a cotton swab into some alcohol and rub it over the slide in a circular motion. Place a drop of water on the center of the slide Step 2 to preparing a wet mount slide. Objects magnified under compound microscopes are mounted onto. This will draw out some water. Then time to place the prepared slide on the microscope bench. Use the edge of this slide to touch the drop. 2. Video of the Day Step 2 Suck up a few drops of your liquid specimen into a medicine dropper. 3.2, step 2). +V_=o?6C_~~P]c,,[email protected]++'}i+ GQ{Z@O?T F Sc%z ~DrB Pe" GI7M8y e,R ff:[QOv36b2"5O`)`Cftc=jS|1?D/}75~Wo[j%?DamhVqP~'P9#sRjTbhK mm+/ zuj^Wqs>@gezn5>r\BB7{|te=Mau7G=>@k5?G-x5Ihk/m?G{Q#C9"A7^8% Kn8 B8pP{l:b]/?Y?|+Jq8p8p8p%8p8p8p8p8p8p({c_99!$(PjAhGCh3qr #*}+nv-~H70" ;cWP_a 3bv;=}Q_Vn _@_XZ~c73#-c. Privacy Policyby Hayley Andersonat MicroscopeMaster.com All rights reserved 2010-2021, Amazon and the Amazon logo are trademarks of Amazon.com, Inc. or its affiliates. Be sure to adjust the focus in order to get a clear view. endstream endobj startxref * How to Prepare a Wet Mount Microscope Specimen * Step One: Obtain a clean microscope slide. Now using the skill of constructing a wet mount, follow the steps above to help you. There are different types of microscopes and also different types of samples, so there is more than one way to prepare a microscope slide. As a small thank you, wed like to offer you a $30 gift card (valid at GoNift.com). At an angle, place one side of the cover slip against the slide making contact with outer edge of the liquid drop. To one sample area of the slide, add a drop of KOH Coverslip both sample areas and examine under a microscope. How to Prepare Microscope Slides. Step-by-step video and audio instructions on how to prepare a wet mount specimen of eukaryotic animal cells; specifically epithelial cells from the inside of the cheek. Place the swab on a glass slide and spread the sample out. field microscopy, differential interference Id]Hvm>s&;`1TtH Take a second clean slide. The general procedure of making a wet mount Place a drop of water on the center of the slide. Stains are used to help identify different types of cells using light microscopes. 18 terms. A cover slip may be used to keep the specimen in place and to help protect the objective lens. Step 3 Pick up a flat glass slide with one hand, grasping it by the outer edges. Sterile microscope slides 5. Using a microscope, observe the wet mount slide. Glass Slides B. Place a sample on the slide. Make a wet-mount preparation of the Staphylococcus culture, following the same procedures just described. Here are the steps on how to make a wet mount slide: Get a piece of clean flat or concave glass slide, and lay it carefully on an even surface, or hold it gingerly at the outer edges. The fluid should not leak out from under the edges of the cover glass. History of the Microscope: Ancient and Medieval History, mount samples, pipette into wells or use prepared slides, Check out anINNOVATIVE NEW MICROSCOPE SLIDE FOR OBSERVING SPECIMENS NOW AVAILABLE -LiveSlide proving to unmask features of microorganisms, Deltaproteobacteria - Examples and Characteristics, Chemoorganotrophs - Definition, and Examples, Betaproteobacteria Examples, Characteristics and Function, Enables researcher to monitor and communicate area(s) of interest, Side-by-side comparisons, including sample to control, Pipe a liquid sample such as blood or slime onto a slide, Using the edge of the second slide, slowly smear the sample creating a thin, even coating, Put a cover slip over the sample, careful not to trap air bubbles, Add a drop of staining solution on the edge of one side of the cover slip, Position the edge of a paper towel on the opposite end, Allow dye to be pulled across the specimen. Especially useful for educational purposes and for those who do not want to undertake the laborious process of creating slides, prepared microscope slides are available in all areas of science, including: if(typeof ez_ad_units!='undefined'){ez_ad_units.push([[728,90],'microscopemaster_com-large-mobile-banner-2','ezslot_17',142,'0','0'])};__ez_fad_position('div-gpt-ad-microscopemaster_com-large-mobile-banner-2-0');Pathology. Your browser doesn't support HTML5 video. Repeat this process two more times for a total of four coats. Your prepared slide is now ready to be inspected beneath a microscope. This is the "wet" part of the wet mount. What problems to avoid when making a wet mount slide? If examining a plant cell, tap water can be used. Carefully place the cover slip on top of the liquid drop. Increase the magnification and adjust the focus to view the sample in greater detail. Wet-mount Slides A wet-mount slide is when the sample is placed on the slide with a drop of water and covered with a coverslip, which holds it in place through surface tension. Your coverslip is now sealed with nail polish. Place the specimen on a clean slide, and if necessary, add a drop of immersion oil. Make notes of any observations made during the examination. "I am giving a local talk on microscopy and was looking for a good basic reference to get people started. Starting with low power, focus on the hair using the proper procedure that you have practised. ThoughtCo. Allow the bacteria to settle on the slide. to be damaged by pressing down too hard on the coverslip, keep the chamber The cover also serves to protect the objective lens from interfering with the sample drop. Mix it properly and apply a coverslip over a uniform suspension without creating bubbles. Consider renting or purchasing an HD camera with a microphone, a computer for editing, a tripod and lighting, and any costumes or props that are needed. Steps in the procedure: 1. Some cells and tissues cannot adhere to a plain glass surface and require a positive charge or surface modifications. Use the footage, sound, and music to create a final cut. Check for the presence of yeast, bacteria, trichomonas and other organisms. A wet mount is like a sandwich. How to Prepare a Wet Mount Specimen Step One - Obtain a clean microscope slide. It will stain carbohydrates in plants and animal specimens brown or blue-black. Place a small drop of a liquid sample onto the slide. How to Grow Table Salt or Sodium Chloride Crystals, Surface Tension - Definition and Experiments, How to Do Chromatography with Candy and Coffee Filters, Ph.D., Biomedical Sciences, University of Tennessee at Knoxville, B.A., Physics and Mathematics, Hastings College. Turn off the condenser and return the slide to its original position in the slide holder. if(typeof ez_ad_units!='undefined'){ez_ad_units.push([[250,250],'microscopemaster_com-leader-1','ezslot_13',124,'0','0'])};__ez_fad_position('div-gpt-ad-microscopemaster_com-leader-1-0'); The dry mount is the most basic technique: simply position a thinly sliced section on the center of the slide and place a cover slip over the sample. Press down the edges to seal the chamber against By signing up you are agreeing to receive emails according to our privacy policy. FB/0~*SGF#YYnUoO Use tweezers to position the specimen in liquid. Never drop slides or slide covers, and set them down only on clean countertops. 1. Place a cover slip over the sample, taking care not to trap air bubbles. It should be about 2 cm2. 1D0Hvv%XM]Tt+ojT.34% Wet mount means placing a sample in drop of water on slide and then covering it with cover slip. This will drain some water out of the system. Take photographs of the specimen and make special note of any abnormal or unusual findings. Surfaces treated with biological reagents can make a slide water-proof, resistant to certain chemicals and reduce instances of cross-contamination. Label the slide with the name of the specimen, the date and your initials. To prepare a wet mount you need a few materials. Collect a drop of stain with an eye dropper or pipette. Place one drop of the liquid specimen in the medicine dropper on top of the slide. It may be necessary to use a knife or razor blade to shave a section of the sample. * Staining Bacteria *. Adjust the microscope's focus knobs until the cells are clearly visible. Why would use a wet mount? Place a sample on the slide. Pick up a flat glass slide with one hand, grasping it by the outer edges. If viewing a sample not already in the liquid, use tweezers to position the specimen within the drop. Place a drop of fluid in the middle of the slide (e.g., water, glycerin, immersion oil, or a liquid sample). Slip Covers C. PipetD. Place the slide under the microscope and adjust the settings to observe the sample. Select the specimen you wish to examine, or prepare a sample if needed. Using a dampened cotton swab, apply pressure to the cover slip to remove any air bubbles. Thanks for the criticism. Deltaproteobacteria is a large group (Class) of Gram-negative bacteria within the Phylum Proteobacteria. Mount the specimen on a concave slide if youre concerned that the specimen will roll or slide off the flat slide. Step 1 to preparing a wet mount slide. Use your free hand to carefully pick up a cover slip (they are extremely fragile). This will help draw the stain under the cover and across the specimen. Obtain a clean microscope slide and a cover slip. Images are used with permission as required. Smooth and pat down the sides of the mound to ensure that it is even and uniform. Place the slide on the microscope stage and lower the stage until the specimen comes into focus. contrast. Wet mount means placing a sample in drop of water on slide and then covering it with cover slip. First, wear the groves. Iodine or methylene blue can be purchased at any education store or biology supply store. Model: ADC H3-8FP: Hyperion 3 3003 Aluminum, NSF approved cookware. The consent submitted will only be used for data processing originating from this website. > Add a drop of physiological saline to the c View the full answer Transcribed image text: Place a drop of fluid in the center of the slide. What is the advantage of using a wet mount preparation? For example, scientists and health professionals use Methylene Blue, a slightly alkaline stain, to reveal the presence of deoxyribonucleic acid, more commonly known as DNA. The bottom layer is the slide. Helmenstine, Anne Marie, Ph.D. "How to Prepare Microscope Slides." Wet mounts are used for living samples, transparent liquids, and aquatic samples. These more expensive microscope slides can be used without a cover.if(typeof ez_ad_units!='undefined'){ez_ad_units.push([[300,250],'microscopemaster_com-medrectangle-4','ezslot_5',114,'0','0'])};__ez_fad_position('div-gpt-ad-microscopemaster_com-medrectangle-4-0'); Some manufacturers produce plastic chambers with a predetermined number of slides with covers. Put down the medicine dropper. Were committed to providing the world with free how-to resources, and even $1 helps us in our mission. For making a wet mount you will need:A. _a#Pi\gm2B=fo[N t!u=Qol~pC?G?2?Hkx Add a small drop of stain to an edge of the coverslip. Last Updated: October 21, 2021 What is the black stuff in Brita water filters? Multiple methods of preparation allow for advanced viewing of inorganic and organic objects.. The cover slip simply sits on top of the specimen. Microscopes are designed to use these slides, and won't work if the slide isn't prepared properly. At this point, either allow the slide to dry so that it can be stained or else place a coverslip on top of the smear. Basic Techniques of I 2. This is the wet part of the wet mount. Which of the following would be the most appropriate steps in creating a wet mount of cheek cells? This is the slide . Add a drop of water onto the slide. Use a flat glass slide to prepare a wet mount. MicroscopeMaster is not liable for your results or any It is a primary test that quickly and cost effectively divides bacteria into one of two types: Gram positive or Gram negative. If the sample is soft, a "squash slide" may be made by. Place one side of a coverslip at an angle so that its edge touches the slide and the outer edge of the drop. Apply gentle heat by passing the slide over a Bunsen burner 3-4 times in . d$oH7q|e70(73_>u.\Y'6{DRmP%i6 Using a pipette, place a drop of water on the specimen. Organisms such as protozoa may only live 30 minutes under a wet mount slide; applying petroleum jelly to the outer edge of the cover slip creates a seal that may . Lower the cover glass onto the water drop at an angle. HV_o0G;#!I;uZM8RB2vghW5Mv6;/ |ox\(-}O ::(5hm|{` v`:T}GNf26v$dI h'ir bM6]2DGhLt6P8RN24hLkHD"(DX|Ffq/ 91L~;#zg]a6u2wOoc}T#U`:U[.KT=^{)S.1jxdC93/[7,,? Apply a thin layer of polish to the underside of the coverslip. Using a sterilized loop, suspend some of the particles in the urine sample onto the slide. M for the first step followed by o for the second step and U, N, and T for the next steps. Microscopy Lab. 225 0 obj <> endobj Apply a second thin layer of clear nail polish over the coverslip. illuminator bulb, usually because the specimen dries up. 2.Using a transfer pipette, obtain a drop of specimen and place onto the center of the slide. Morello-Mizer-Granato: I I. Additional variations to microscope slides include: if(typeof ez_ad_units!='undefined'){ez_ad_units.push([[580,400],'microscopemaster_com-banner-1','ezslot_7',111,'0','0'])};__ez_fad_position('div-gpt-ad-microscopemaster_com-banner-1-0'); Almost always made of borosilicate or silicate glass, cover slips hold samples in place and protect them from inadvertent movement and contamination. First I used paper to represent the specimen. Place the steps in the correct order. Return from Microscope Slides to Best Microscope Homeif(typeof ez_ad_units!='undefined'){ez_ad_units.push([[728,90],'microscopemaster_com-leader-2','ezslot_18',143,'0','0'])};__ez_fad_position('div-gpt-ad-microscopemaster_com-leader-2-0'); Find out how to advertise on MicroscopeMaster! The following paragraphs describe the major steps in preparing paraffin sections. \8)SBI Step 1: Materials 2 More Images For making a wet mount you will need: A. Draw what you see. Turn on the condenser and adjust the light intensity and focus as needed. Write the script and create actors or characters to play the roles. h` ~ Place one side of the cover slip on slide at the edge of the concave center 4. Your objective is to have sufficient water to fill the space between cover slip and slide. Place the slide on a flat surface and carefully place one drop of water on the slide. When it is complete, look at it under the microscope. If there is too much water, the cover slip will slide around. % of people told us that this article helped them. She received her MA in Environmental Science and Management from the University of California, Santa Barbara in 2016. This will minimize disturbing air bubbles. Gather all of the necessary materials and equipment needed to make a film. Slowly lower the other edge of the cover slip until it rests on the . Each type of preparation method is used for mounting different types of cells. hb``g``j````6; Advantages This type of slide preparation allows you to view microscopic living things without them drying out. Place a drop of the prepared specimen on the slide. take the utmost precaution and care when performing a microscope Cheek cells can be easily obtained by gently scraping the inside of the mouth using a clean, sterile cotton swab. 3.) To prepare a wet mount using a flat slide or a depression slide: Some living organisms move too quickly to be observed in a wet mount. Position sample on liquid, using tweezers. Make sure there are no air bubbles trapped underneath. Place a coverslip on top of the specimen. Place the cover slip over the sample, making sure the edges align. Figure 1: A diagnostic section being prepared with a cryostat microtome. One solution is to add a drop of a commercial preparation called "Proto Slow." Select a microscope slide and place it flat on a clean surface. Place a drop of water on a clean microscope slide. There are many methods of staining slides. Continue with Recommended Cookies. Observe preparation under the 40X objective lens. To prepare a slide from cells grown on an agar medium, first place a SMALL drop, a loopful works well, of water on a clean grease-free slide. What are the three steps to making a wet mount slide? The Hyperion3 collection is constructed of premium 3003 aluminum for superior heat transfer. 2.) Prepare a wet mount with those cells Remove the skin of the provided onion and carefully slice a small specimen to observe. In some cases, it's okay to view the sample without a coverslip, as long as care is taken not to bump the sample into the microscope lens. Put the glass slide on your microscope and lay the specimen in the middle. Ideally, two smears can be prepared on one slide, of which one can be stained with iodine. Water E. Specimen ( It is the middle of winter I couldn't get a leaf so I used a piece of paper). This article has been viewed 86,883 times. We have tried a new approach to routine stool microscopy by using a combination of methylene blue and glycerol in wet mount preparation of fresh faecal samples for the demonstration of medically important intestinal parasites. Be sure to set up proper camera angles and lighting, capture good sound, and have a plan for filming each scene. Wet Mount: Place a drop of fluid in the center of the slide. In order to heat fix a bacterial smear, it is necessary to first let the bacterial sample air dry. Do not tap or press on the cover slip once it is in place. Although wet mounts can be used to prepare a significantly wide range of microscope slides, they provide a transitory window as the liquid will dehydrate and living specimens will die. Obtain a clean microscope slide and cover slip. Preparing a Wet Mount Introduction The Wet Mount is a procedure performed in the laboratory to observe motile organisms. Once the slide is heat fixed, it can then be stained. HVn0}7T6l@an\5wA~f[cf4#CG:&6#]Aa0K,zJ ia# Scrape inside of cheek with toothpick (firmly but gently). Take a clean and grease-free slide. Helmenstine, Anne Marie, Ph.D. (2020, August 28). The following steps are used to prepare a wet mount slide. Since they are used for primarily inorganic and dead matter, dry mounts can theoretically last indefinitely. Although wet mounts can be used to prepare a significantly wide range of microscope slides, they provide a transitory window as the liquid will dehydrate and living specimens will die. Press gently on the coverslip to remove air bubbles and seal the slide. Put a drop of stain on an outer edge of your cover slide. Pull a strand of hair from your head and only your head and place it on the slide. Suited for high-resolution microscopy and oil immersion preparations, Number 1 covers are .13-.17mm thick. Common Objects to Examine With a Microscope. Share it with us! ** Be sure to Hold it at an angle to the first slide. This will usually take a few minutes. Great job. Students also viewed. Make sure the drop goes on the center portion of the slide. 3.2, 78 Steps Health Weight Loss (current) Blood Sugar Premier Spinal Force Chinese Pain Reliever If too dry, add a drop of water beside the cover slip. 6 How do you make a wet mount microscope slide? JavaScript seems to be disabled in your browser. step 3: Obtain a clean microscope slide and cover slip. Whether you mount samples, pipette into wells or use prepared slides, microscope slides offer a vehicle to contain a specimen while observing under magnification. H]K02%i Develop the tech skills you need for work and life. They will also stain skin (temporarily) and clothing (permanently), so wear clothes that you dont care about when handling the chemicals. They are more expensive and usually used without a cover slip. To use one of these stains: Many common foods and objects make fascinating subjects for slides. Method 1 Preparing a Dry Mount 1 Select a clean slide. yz?th46l.Q3 gmomM~k#S You may need to add another drop to ensure complete coverage. The slide should be clean and free of dust or other fine particles. Place a drop of liquid or solution onto the slide. Another problem is that large bubbles may lower the viewing resolution. Create a detailed storyboard, which will include all of the scenes, shots, and transitions that will be necessary for the film. 1. Place the cover slip at an angle over the sample so that it does not touch the drop of the sample. Standard slides can be either plastic or glass and are 1 x 3 inches (25 x 75 mm) in size and 1 to 1.2 mm thick. 3.2, step 2). Sharps container 3. Then lower the cover slip completely. Simply take a single cover slip and hold it between thumb and forefinger by the edges. Adding a couple of strands of cotton from tissue or swab or else adding tiny bits of broken coverslip will add space and "corral" the organisms. I am. The slide should be clean and free of dust or other fine particles. 2 See answers Advertisement S::efB';=5(`n]V#xPLc Gently lower the slide onto a microscope stage. Then either place the slide in the slide holder of a microincinerator, or pass the dried slide through the flame of a Bunsen burner 3 or 4 times, smear side facing up.
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